-GFPHuman mammary artery endothelial cells GFP labeling-

GFP-HIMAECs were selected from HIMAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels > 16 when cultured following the detailed protocol described below.

Medium Formulation:

ECGM Endothelial Cell Culture Medium (P1001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing: 5% DMSO+95% FBS

Cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

For research use only