-RFPBovine aortic endothelial cells RFP labeling-

RFP-bAECs are selected from bAECs transfected using lentivirus expressing RFP using Puromycin. The cells are shipped in a frozen vial(the cells are provided @ passage 2). Endothelial Growth Medium is recommended for the expansion of RFP-bAECs and these cells can be propagated to at least 12 population doubling times without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below.

Medium Formulation:

ECGM endothelial cell culture medium (P1001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

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