BALB/cSpleen cells of BALB / c mice-

Mouse primary spleen cells (MPSPLCs) are isolated from tissue of pathogen-free laboratory mice. MPSPLCs are mixed population of mouse spleen cells grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Medium Formulation:

RPMI-1640（MD0200）+10% FBS（SD0200）+2 mM L-谷氨酰胺（SD0007）+10 mM HEPES+P/ S（SD0005）

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

For research use only