OCT3/4 Expressed By Human Mesenchymal Stem Cells

Background: It Is Well Established That Induced Pluripotent Stem Cells May Be Generated By Co Transfer Of Four Genes: Oct3/4, Sox2, Kfl4 And C-Myc, Into Soft Adult Cells Resulting In Re Programming Of These Cells To Pluripotency Equivalent To That Of Empirical Stem Cells It Has Recently Been Shown By Independent Research Including Results From Our Laboratory That Over-expression Of A Single Gene, Oct3/4, In Adult Neural Or Mesenchymal Stem Cells Also Results In Enhanced Differentiation Capacity Including Pluripotency.  This Development Simplifies IPS Generation And May Reflect Levels Of Endogenous Expression Of Sox2, Kfl4 And C-Myc In Adult Stem Cells That Are Multipotent.Also, Since The Oct3/4 Promoter Has Well-known Response Elements And Transcription Control Mechanisms,  Re-programming Based On Environmental Cues Is Now Feasible Through Use Of Small Molecular Agents That Selectively Activate Expression Of Oct3/4.  Our Human MSCs Expressing Oct3/4 Allow Further Investigation Of IPSC Mechanisms.Optimizing Your HMSC Cultures:  1) Grow Cells In 1 To 5% O2, 5% CO2, With The Balance Of The Gas Content As N2.. 2) Use Of Our MSCGroTM Media Is Highly Recommended. 3) Subtract - We Recommended Greiner Bio One TC Created Cell Culture Flies Or Equivalent, It Has The Same Pluripotency As Embryonic Stem Cells. Recently, Independent Studies Including Our Laboratory Results Have Shown That Overexpression Of A Single Gene Oct3/4 In Adult Neural Or Mesenchymal Stem Cells Can Also Lead To Enhanced Differentiation, Including Pluripotency. This Progress Simplifies The Production Of IPS And May Reflect The Endogenous Expression Level Of Sox2, Kfl4 And C-Myc In Pluripotent Adult Stem Cells. In Addition, Because The Oct3/4 Promoter Has Well-known Response Elements And Transcriptional Control Mechanisms, Reprogramming Based On Environmental Cues Is Now Feasible By Using Small Molecule Reagents To Selectively Activate The Expression Of Oct3/4. Our Human MSCs Expressing Oct3/4 Can Further Study The IPSC Mechanism. Optimize Your HMSC Culture: 1) Cultivate Cells In 1 - 5% O2 And 5% CO2, And Balance The Gas Content With N2. 2) MSCGroTM Media Is Highly Recommended. 3) Reduction - We Recommend Greiner Bio One Tc Treated Cell Culture Bottles Or Equivalent. Animal Species/Oganism & Nbsp; People Organization Source; Tissue And Cell Type  Morphology Of Pluripotent Stem Cells; Wall Attached Growth& Nbsp; Note: This Product Is Only For Scientific Research