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Human Caucasian colon adenocarcinoma. Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.
CSAp negative (CSAp-); Colon antigen 3, positive; The cells are positive for keratin by immunoperoxidase stainingTumorigenecity: yes, in nude miceIsoenzyme: G6PD, B;PGM1, 1;PGM3, 1-2;6PGD, A;ES-D, 1;PEP-D, 1Histopathology: adenocarcinoma; Grade IIISubculture: Split ratio: Media change: Reverse transcritase: Productioncarcinoembryonic antigen(CEA) 155ng/10 exp6 cells/10days; Keratin.